evaluation of morphophysiological traits of cotton cultivars (gossypium hirsutum) under water deficiency stress at seedling stage
نویسندگان
چکیده
introduction major cultivated cotton regions of iran are located in dry and semiarid climates, therefore water deficiency or drought stress is inseparable part of cotton production systems in these regions. so identification and introduction of drought tolerant cotton genotypes is crucial. showed that water stress decreased plant growth rate, leaf area and finally photosynthesis in cotton. in addition plant height reduction is a primary effect of water stress. according to results, net photosynthesis, stomatal conductance and transpiration decreased simultaneously with an increase in drought stress intensity. physiologic traits monitoring were notified as a proper gadget for selection and improvement of germplasm. whereas investigation of many genotypes in field conditions under drought stress is difficult and it is not accurate enough, also good correlation has observed between the results of drought tolerance at seedling stage and field experiments, therefore this research has tried to evaluate some of the morphologic traits in cotton genotypes at seedling stage under drought stress and none stress conditions. materials and methods 22 cotton cultivars were grown under none water stress (field capacity) and drought stress conditions (-1mpa) using a factorial arrangement of treatments based on randomized completely design with three replications at college of agriculture, ferdowsi university of mashhad in 2011. then each genotype (10 seed) was sown at a pot (1.5 l-1). at two true leaf stages, pots were tinned to one seedling. soil moisture content was kept up to two true leaf stages at field capacity and at the end of experiment, it was conserved about -1mpa using weight method. the measured parameters were: plant height, leaf area, dry weight of leaf, stem, root and whole plant and also decreasing percent of each parameter under stress in comparison with control in any genotype was determined at the end of experiment. stomatal resistance and leaf temperature were measured with leaf porometer set (model decagon devices, inc) on three leaf stage of cotton seedling. relative water content (rwc) of leaf was also measured. variance analysis, comparison of trait means and correlation between traits were carried out using sas and excel and least significant difference (lsd). results and discussion interaction between water deficiency stress and cotton cultivars were significant (p<0.01). drought stress decreased the number of leaf per plant significantly (p<0.01) by about 43.2%. leaf area decreased significantly (p<0.01) under drought stress, but there was not significant correlation between leaf area and total dry mater or leaf dry weight. this object showed that effect of variations of leaf area on the amount of drought tolerance in relation with dry matter production was low. overall drought stress decreased dry weight of stem (29.1%), leaf (59.9%) and root (61.5%) significantly at the all cultivars in comparison with well-watered condition. according to the correlation coefficient, leaf dry weight altered with changes in leaf surface (r=0.82**) and leaf number (r=0.76**). under conditions (stress and none stress), maximum dry matter had allocated in leaves. in addition to cultivars such as varamin and 43347 that had the lowest decrease in leaf area and leaf and stem dry weight, root dry weight showed the lowest decrease of. the most decrease of plant dry weight after drought stress belonged to 43259 (80.7%), asj2*349 (72.5%) and nazili84 (72.1%) cultivars and the least decrease of plant dry weight after drought stress belonged to varamin cultivar (39.7%). according to the correlation coefficients, changes of leaf dry weight (r=0.95**), stem dry weight (r=0.94**) and leaf number per plant (r=0.80**) had the most effect on changes of plant dry weight. the results showed that cultivars with the maximum dry weight necessarily had not the lowest decrease of dry weight in comparison with the control. according to the result of leidi and et al, (1999) drought stress decreased relative water content of leaf in cotton cultivars significantly (p<0.01), tolerant cotton cultivars showed lower decreasing of rwc under drought stress in comparison with susceptible cultivars and also plants with higher rwc had higher plant dry weight. drought stress had significant effect (p<0.01) on stomatal resistance and amount of stomatal resistance reached 4.8 fold under drought stress. cotton cultivars showed obvious difference in stomatal resistance. 43347 and nazili84 cultivars had the highest and varamin and irma had the lowest stomatal resistance, respectively. in addition stomatal resistance increased more in susceptible cotton cultivars in comparison with tolerant cotton. stomatal resistance had negative effect on other parameters. drought stress increased leaf temperature significantly (p<0.01) and also difference of leaf temperature among cotton cultivars was significant (p<0.01)
منابع مشابه
Physiological Mechanism of Nitrogen Mediating Cotton (Gossypium hirsutum L.) Seedlings Growth under Water-Stress Conditions
The objective of this investigation was to study the effects of nitrogen on tolerance to water-stress in cotton (Gossypium hirsutum L.) seedlings. Growth chamber studies with pots of washed sand were carried out in Fayetteville, USA, and Nanjing, Chinawith three water conditions (well-watered, drought-stressed, and waterlogging), and three nitrogen rates, low nitrogen (16 mM, approximately 224 ...
متن کاملRNA-Seq Transcriptome Profiling of Upland Cotton (Gossypium hirsutum L.) Root Tissue under Water-Deficit Stress
An RNA-Seq experiment was performed using field grown well-watered and naturally rain fed cotton plants to identify differentially expressed transcripts under water-deficit stress. Our work constitutes the first application of the newly published diploid D5 Gossypium raimondii sequence in the study of tetraploid AD1 upland cotton RNA-seq transcriptome analysis. A total of 1,530 transcripts were...
متن کاملEffect of Nitrogen on Seed Cotton Yield and Fiber Qualities of Cotton (gossypium Hirsutum L.) Cultivars
Seed cotton yield and fiber qualities may be significantly altered by a number of agronomic practices. The present study investigates the effect of nitrogen levels on cotton cultivar's seed cotton yield and fiber quality traits. Three cotton cultivars (NIAB-111, CIM496 and FH901) were tested against four nitrogen levels (0, 60, 120 and 180 kg ha) to check reliability, the highest yielding and g...
متن کاملAscorbate Alleviates Fe Deficiency-Induced Stress in Cotton (Gossypium hirsutum) by Modulating ABA Levels
Fe deficiency causes significant losses to crop productivity and quality. To understand better the mechanisms of plant responses to Fe deficiency, we used an in vitro cotton ovule culture system. We found that Fe deficiency suppressed the development of ovules and fibers, and led to tissue browning. RNA-seq analysis showed that the myo-inositol and galacturonic acid pathways were activated and ...
متن کاملGenotypic variability for root/shoot parameters under water stress in some advanced lines of cotton (Gossypium hirsutum L.).
Research pertaining to genetic variability parameters, heritability, and genotypic, phenotypic, simple, and environmental correlations for various seedling traits in five elite advanced cotton (Gossypium hirsutum L.) lines (FH-113, FH-114, FH-941, FH-942, and FH-2015) and one check (CIM-496) was carried out during October and November 2010 under greenhouse conditions at the Cotton Research Inst...
متن کاملEvaluation and Exploration of Favorable QTL Alleles for Salt Stress Related Traits in Cotton Cultivars (G. hirsutum L.).
Soil salinization is one of the major problems in global agricultural production. Cotton is a pioneer crop with regard to salt stress tolerance, and can be used for saline-alkali land improvement. The large-scale detection of salt tolerance traits in cotton accessions, and the identification of elite quantitative trait loci (QTLs)/genes for salt-tolerance have been very important in salt tolera...
متن کاملمنابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
عنوان ژورنال:
پژوهش های زراعی ایرانجلد ۱۴، شماره ۳، صفحات ۴۰۳-۰
میزبانی شده توسط پلتفرم ابری doprax.com
copyright © 2015-2023